A solid-phase enzyme-linked assay for vitamin B12
نویسندگان
چکیده
منابع مشابه
A Solid-Phase Enzyme-Linked Assay for Vitamin B12
A new solid-phase enzyme-linked competitive binding assay for vitamin B12 (cyanocobalamin) is described. The assay is based on the competition between analyte B12 molecules and a glucose-6-phosphate dehydrogenase-vitamin B12 conjugate for a limited number of R-protein binding sites immobilized on sepharose particles. After appropriate incubation and washing steps, the enzyme activity bound to t...
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ژورنال
عنوان ژورنال: Mikrochimica Acta
سال: 1989
ISSN: 0026-3672,1436-5073
DOI: 10.1007/bf01197285